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Publications

Barton MI, MacGowan SA, Kutuzov MA, Dushek O, Barton GJ, van der Merwe PA (2021) Effects of common mutations in the SARS-CoV-2 Spike RBD domain and its ligand the human ACE2 receptor on binding affinity and kinetics. (Submitted, biorxiv)

MacGowan SA, Barton MI, Kutuzov M, Dushek O, van der Merwe PA, Barton GJ (2021) Missense variants in human ACE2 modify binding to SARS-CoV-2 Spike. (Submitted, biorxiv)

Goyette J, Depoil D, Yang Z, Isaacson SA, Allard J, van der Merwe PA, Gauss K, Dustin ML, Dushek O (2021) Regulated unbinding of ZAP70 at the T cell receptor by kinetic avidity (submitted, BioRxiv)

Lanz AL, Masi G, Porcello N, Cohen A, Cipria D, Prakaash D, Balint S, Raggiaschi R, Galgano D, Cole DK, Lepore M, Dushek O, Dustin ML, Sansom MSP, Kalli AC, Acuto O (2021) Allosteric activation of T-cell antigen receptor signalling by quaternary structure relaxation. Cell Reports (in press, biorxiv)

*Pettmann J, *Huhn A, *Abu Shah E, Kutuzov MA, Wilson DB, Dustin ML, Davis SJ, van der Merwe PA, Dushek O (2021) The discriminatory power of the T cell receptor. eLife (see associated Press Release)

Clemens L, Kutuzov M, Bayer KV, Goyette J, Allard J, Dushek O (2021) Determination of the molecular reach of the protein tyrosine phosphatase SHP-1. Biophysical Journal

Clemens L, Dushek O, Allard J (2020) Intrinsic disorder in the T cell receptor creates cooperativity and controls ZAP70 binding. Biophysical Journal

*Trendel N, *Kruger P, Nguyen J, Gaglione S, Dushek O (2021) Perfect adaptation of CD8+ T cell responses to constant antigen input over a wide range of affinity is overcome by costimulation. Science Signalling

Salzer B, Schüller C, Brey CU, Peters T, Schöber M, Lobner E, Dushek O, Huppa J, Holter W, Traxlmayr MW, Lehner M (2020) AvidCARs – a novel platform for integration of multiple input signals by one CAR molecule. Nature Communications

Abu-Shah E, Trendel N, Kruger P, Nguyen J, Pettmann J, Kutuzov M, Dushek O (2020) Human CD8+ T cells exhibit a shared antigen threshold for different effector responses. Journal of Immunology.

Paoletti F, El-Sagheer A, Allard J , Brown T, *Dushek O, *Esashi F (2020) Molecular flexibility of DNA as a major determinant of RAD51 recruitment. EMBO Journal (Highlighted with News & Views)

Zhang Y, Clemens L, Goyette J, Allard J, *Dushek O, *Isaacson SA (2019) The influence of molecular reach and diffusivity on the efficacy of membrane-confined reactions. Biophysical Journal

Abu-Shah E, Demetriou P, Mayya V, Balint S, Kutuzov MA, *Dushek O, *Dustin ML (2019) A tissue-like platform for studying engineered quiescent human T-cells' interactions with dendritic cells. eLife

Knapp B, van der Merwe PA, Dushek O, Deane CM (2019) MHC binding affects the dynamics of different T-cell receptors in different ways. PLOS Computational Biology

Limozin L, Bridge M, Bongrand P, Dushek O, van der Merwe PA, Robert P (2019) TCR-pMHC kinetics under force in a cell-free system show no intrinsic catch bond, but a minimal encounter duration before binding. PNAS (Commentary by Schutz & Huppa)

Fernandes RA*, Ganzinger KA*, Tzou J, Jönsson P, Lee SF, Palayret M, Chang VT,  Santos MDC, Macleod C, Lindsay AE, Dushek O, Tilevik A, Davis SJ, Klenerman D (2019) Topological constraints on close-contact formation that limit CD45 exclusion provide a mechanism for discriminatory T-cell receptor signalling. PNAS

Dushek O, Dustin ML (2018) Thymocyte selection: CD8 helps TCR catch slippery self pMHC. Nature Immunology

Pettmann J, Santos AM, *Dushek O, *Davis SJ (2018) Membrane ultrastructure and T-cell activation. Frontiers in Immunology

Farfán JAS, Dushek O (2018) Molecular mechanisms of T cell sensitivity to antigen. Immunological Reviews

Harris DT, Smith SN, Cai Q, Stone JD, Kruger P, Lever M, Dushek O, Schmitt TM, Greenberg PD, Kranz DM (2018) Comparison of T cell activities mediated by human TCRs and CARs that use the same recognition domains Journal of Immunology

*Pan W, *Yang W, *Chen S, *Trendel N, Xiao F, Wu W, Xue M, Li X, Ji H, Liu X, Jiang H, Wang H, Shen H, *Dushek O, *Li Hua, *Xu C (2017) CD28 signaling is regulated by a dynamic electrostatic environment. Nature Structural & Molecular Biology

Goyette J, Solis-Salas C, Coker-Gordon N, Bridge M, Isaacson SA, Allard J, Dushek O (2017) Biophysical assay for tethered signalling reactions reveals tether-controlled activity for the phosphatase SHP-1. Science Advances

Lever M, Lim HS, Kruger P, Nguyen J, Trendel N, Abu-Shah E, Maini PK, van der Merwe PA, Dushek O (2016) Architecture of a minimal signalling pathway explains the T cell response to a 1,000,000-fold variation in antigen affinity and dose. PNAS

Azad A, Lim SY, D'Costa Z, Diana A, Sansom OJ, Kruger P, Liu S, McKenna WG, Dushek O, Muschel RJ, Fokas M (2016) PD-L1 blockcade enhances responses of pancreatic ductal adenocarcinoma to radiotherapy. EMBO Molecular Medicine (News & Views by Jan Dorrie)

Banerji S, Lawrance W, Metcalfe C, Briggs DC, Yamauchi A, Dushek O, van der Merwe PA, Day AJ, Jackson DG (2016) Homodimerisation of the Lymph Vessel Endothelial Receptor LYVE-1 through a Redox-Labile Disulfide is critical for Hyaluronan Binding in Lymphatic Endothelium. Journal of Biological Chemistry

Zhang H, Lim HS, Knapp B, Deane CM, Aleksic M, Dushek O, van der Merwe PA (2016) The contribution of major histocompatibility complex contacts to the affinity and kinetics of T cell receptor binding. Scientific Reports.

Mukhopadhyay H , de Wet B , Clemens L , Maini PK, Allard J, van der Merwe PA, Dushek O  (2016) Multisite phosphorylation of the T cell receptor ζ-chain modulates potency but not the switch-like response. Biophysical Journal. (New & Notable by Faeder & Morel)

Jönsson P,  Southcombe JH, Santos AM, Huo J, Fernandes RA, McColl J, Lever M,  Evans EJ, Hudson A, Chang VT, Hanke T, Godkin A, Dunne PD, Horrocks MH, Palayret M, Screaton G, Petersen J, Rossjohn J, Fugger L, Dushek O, Xu XN, Davis SJ, Klenerman D (2016) The remarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions. PNAS

Lim HS, Cordoba SP, Dushek O, Goyette J, Taylor A, Rudd CE, van der Merwe PA (2015) Costimulation of IL-2 Production through CD28 Is Dependent on the Size of Its Ligand. Journal of Immunology

Paster W, Bruger AM, Katsch K, Grégoire C, Roncagalli R, Fu G, Gascoigne NR, Nika K, Cohnen A, Feller SM, Simister PC, Molder KC, Cordoba SP, Dushek O, Malissen B, Acuto O (2015) A THEMIS:SHP1 complex promotes T-cell survival. EMBO Journal

Lever M, Maini PK, van der Merwe PA, Dushek O (2014) Phenotypic models of T cell activation. Nature Reviews Immunology (phenotypic model animations for ligands with short, intermediate, and long dissociation times)

Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, Dushek O, van der Merwe PA, Howarth M (2014) SpyAvidin hubs enable precise and ultrastable orthogonalnanoassembly. J Am Chem Soc.

Dushek O, Lellouch AC, Vaux DJ, Shahrezaei V (2014) Biosensor architectures for high-fidelity reporting of cellular signaling. Biophysical Journal

Dushek O, van der Merwe PA (2014) An induced rebinding model of antigen discrimination. Trends in Immunology

Salio M, Ghadbane H, Dushek O, Shepherd D, Cypen J, Gileadi U, Aichinger MC, Napolitani G, Qi X, van der Merwe PA, Wojno J, Veerapen N, Cox LR, Besra GS, Yuan W, Cresswell P, Cerundolo V (2013) Saposins modulate human invariant Natural Killer T cells self-reactivity and facilitate lipid exchange with CD1d molecules during antigen presentation. PNAS

Mukhopadhyay H, Cordoba SP, Maini PK, van der Merwe PA, Dushek O (2013) Systems model of T cell receptor proximal signaling reveals emergent ultrasensitivity. PLOS Computational Biology

Salek M, McGowan S, Trudgian DC, Dushek O, de Wet B, Efstathiou G, Acuto O (2013) Quantitative phosphoproteome analysis unveils LAT as a modulator of CD3ζ and ZAP-70 tyrosine phosphorylation. PLOS One

Dushek O, Goyette J, van der Merwe PA (2012) Non-catalytic tyrosine-phosphorylated receptors. Immunological Reviews

Allard JF, Dushek O, Coombs D, van der Merwe PA (2012) Mechanical modulation of receptor-ligand interactions at cell-cell interfaces. Biophysical Journal

Robert P, Aleksic M, Dushek O, Cerundolo V, Bongrand P, van der Merwe PA (2012) Kinetics and mechanics of two-dimensional interactions between T cell receptors and different activating ligands. Biophysical Journal

Dushek O (2012) Elementary steps in T cell receptor triggering. Frontiers Immunology

Zhang H, Cordoba SP, Dushek O, van der Merwe PA (2011) Basic residues in the T-cell receptor ζ cytoplasmic domain mediate membrane association and modulate signaling. PNAS

*Dushek O, *Aleksic M, Wheeler RJ, Zhang H, Cordoba SP, Peng YC, Chen JL, Cerundolo V, Dong T, Coombs D, van der Merwe PA (2011) Antigen potency and maximal efficacy reveal a mechanism of efficient T cell activation. Science Signalling.

Dushek O, van der Merwe PA, Shahrezaei V (2011) Ultrasensitivity in multisite phosphorylation of membrane-anchored proteins. Biophysical Journal

van der Merwe PA, Dushek O (2011) Mechanisms for T cell receptor triggering. Nature Reviews Immunology.

Nika K, Soldani C, Salek M, Paster W, Gray A, Etzensperger R, Fugger L, Polzella P, Cerundolo V, Dushek O, Höfer T, Viola A, Acuto O (2010) Constitutively active Lck kinase in T cells drives antigen receptor signal transduction. Immunity.

Treanor B, Depoil D, Gonzalez-Granja A, Barral P, Weber M, Dushek O, Bruckbauer A, Batista FD (2010) The membrane skeleton controls diffusion dynamics and signaling through the B cell receptor. Immunity.

*Aleksic M, *Dushek O, Zhang H, Shenderov E, Chen JL, Cerundolo V, Coombs D, van der Merwe PA (2010) Dependence of T cell antigen recognition on T cell receptor-peptide MHC confinement time. Immunity. (Preview by Govern & Chakraborty)

Dushek O, Das R, Coombs D (2009) A role for rebinding in rapid and reliable T cell responses to antigen. PLOS Computational Biology

Dushek O, Das R, Coombs D (2008) Analysis of membrane-localized binding kinetics with FRAP. Eur Biophys J.

Dushek O, Coombs D (2008) Analysis of serial engagement and peptide-MHC transport in T cell receptor microclusters. Biophysical Journal

Dushek O, Coombs D (2008) Improving parameter estimation for cell surface FRAP data. J Biochem Biophys Methods.

Dushek O, Mueller S, Soubies S, Depoil D, Caramalho I, Coombs D, Valitutti S (2008) Effects of intracellular calcium and actin cytoskeleton on TCR mobility measured by fluorescence recovery. PLOS One.